• Santiago R Leal-Noval, Victoria Arellano, Alejandro Vallejo, Ana Hernández, Antonio Ordóñez, Rafael Hinojosa, Juan Polo, Manuel Múñoz, and Manuel Leal.
• Critical Care Division, Preventive Medicine and Health Public, University of Seville, Seville, Spain. srleal@telefonica.net
• J. Cardiothorac. Vasc. Anesth. 2009 Jun 1; 23 (3): 330-5.

ObjectiveThe purpose of this study was to evaluate the influence of preoperative type I and II immune responses on blood transfusion requirements.DesignA prospective and observational trial.SettingA postcardiac surgery unit of a university hospital.ParticipantsSeventy-one consecutive patients undergoing elective cardiac surgery.InterventionsBlood samples drawn for laboratory analysis and immunologic study.Measurements And Main ResultsPatients were divided into 2 groups according to blood transfusion requirements: < or = 2 units (n = 35) and >2 units of red blood cells (n = 36). The preoperative immune response was assessed by flow cytometry, measuring the proportion of CD4+ T helper cells producing cytokines, including Th1 response (interferon-gamma and tumor necrosis factor-alpha [TNF-alpha]) and Th2 response (interleukin 4 and 10). Two logistic regression analyses (including and not including immunologic variables) were used to select and weight perioperative variables associated with an increased risk of transfusion. Three variables were found to be independent predictors of transfusion requirements when immunologic variables were not included: preoperative platelet count, preoperative hemoglobin, and hypertension. When all the variables were included, preoperative hemoglobin, cardiopulmonary bypass time, and the preoperative proportion of CD4+ T cells producing TNF-alpha were associated with an increased risk of transfusion (Hosmer-Lemeshow, 0.33; c-index, 0.93), but preoperative platelet count and hypertension were not.ConclusionsA low preoperative Th1 immune response, as assessed by the proportion of CD4+ T-helper-producing TNF-alpha, was associated with a higher blood transfusion rate.

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