• Anesthesiology · Mar 2008

    Comparative Study

    Autologous transplantation of endothelial progenitor cells attenuates acute lung injury in rabbits.

    • Chen-Fuh Lam, Yen-Chin Liu, Jen-Kuo Hsu, Pei-An Yeh, Ting-Ya Su, Chien-Chi Huang, Ming-Wei Lin, Ping-Ching Wu, Pei-Jung Chang, and Yu-Chuan Tsai.
    • Department of Anesthesiology, National Cheng Kung University College of Medicine and Hospital, Tainan, Taiwan.
    • Anesthesiology. 2008 Mar 1; 108 (3): 392-401.

    BackgroundAcute lung injury (ALI) and end-stage acute respiratory distress syndrome (ARDS) are among the most common causes of death in intensive care units. Activation and damage of pulmonary endothelium is the hallmark of ALI/ARDS. Recent studies have demonstrated the importance of circulating endothelial progenitor cells (EPCs) in maintaining normal endothelial function as well as endothelial repairing after vascular injury. Here, the authors present the first study demonstrating the therapeutic potential of EPCs in a rabbit model of ALI/ARDS.MethodsCirculating EPCs were obtained from rabbits using Ficoll centrifugation. One week after culturing, ALI was induced in rabbits by oleic acid (75 mg/kg, intravenous), and autologous EPCs were transplanted intravenously. Vasomotor function of isolated pulmonary artery and degrees of lung injury were assessed 2 days later.ResultsEndothelial dysfunction in the pulmonary artery was significantly attenuated in rabbits treated with EPCs, whereas the endothelium-independent relaxation responses were not different. Expression of inducible nitric oxide synthase was suppressed in the pulmonary artery of EPC-treated animals. Infiltration of leukocytes in the lung parenchyma was significantly reduced after EPC transplantation. EPCs also decreased water content, hyaline membrane formation, and hemorrhage in lungs.ConclusionThe authors demonstrated that autologous transplantation of EPCs preserves pulmonary endothelial function and maintains the integrity of pulmonary alveolar-capillary barrier. Transplantation of EPCs can be a novel cell-based, endothelium-targeted therapeutic strategy for prevention and treatment of ALI/ARDS.

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