• Tomoyo Onuki, Shin Nihonyanagi, Masaki Nakamura, Toshimitsu Ide, Jun Hattori, Yuhsaku Kanoh, and Kazui Soma.
• Department of Clinical Laboratory, Kitasato University Hospital.
• Kansenshogaku Zasshi. 2013 Jan 1; 87 (1): 33-8.

AbstractClostridium tetani is widely distributed in ground or mud, especially in field and pond-shore surface layers. C. tetani is rarely isolated from specimens of patients with tetanus, and is generally diagnosed based on clinical symptoms such as trismus or general tonic spasms. This means that positive C. tetani infection is rarely diagnosed bacterially. Using gram straing, we identified C. tetani in specimens from patients suspected of C. tetani infection brought to the Kitasato University Hospital emergency center. Rapid gram staining information in the bacteriology laboratory is expected to improve recovery from C. tetani infection. It is therefore necessary to ensure clinical specimen quality control, and to keep standard strains of rare bacteria for isolation and identification.

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