• Marta Crespo, Alberto Torio, Virginia Mas, Dolores Redondo, Maria J Pérez-Sáez, Marisa Mir, Anna Faura, Rita Guerra, Olga Montes-Ares, Maria D Checa, and Julio Pascual.
• Department of Nephrology, Hospital del Mar, Paseo Marítimo 25-29, 08003 Barcelona, Spain; Institute Hospital del Mar for Medical Research (IMIM), Paseo Marítimo 25-29, 08003 Barcelona, Spain. Electronic address: mcrespo@hospitaldelmar.cat.
• Transpl. Immunol. 2013 Dec 1; 29 (1-4): 28-33.

UnlabelledAnti-HLA donor-specific antibodies (DSA) identified by single antigen bead array (SAB) are questioned for their excess in sensitivity and lack of event prediction after transplantation.Population And MethodsWe retrospectively evaluated specific types of preformed DSA (class I, class II or C1q-fixing) and their impact on graft survival. Kidney transplantations performed across negative CDC-crossmatch were included (n=355). Anti-HLA antibodies were tested using SAB to identify DSA and their capacity to fix C1q.ResultsTwenty-eight patients with pretransplant DSA(+) with MFI>2000 were selected to assess C1q fixation. DSA were C1q+ in 15 patients and C1q- in 13, without significant differences in demographics, acute rejection, graft loss or renal function. The maximum MFI of DSA in patients with C1q-fixing DSA was significantly higher (p=0.008). Patients with DSA class-I suffered more antibody-mediated rejection (AMR) and had worse graft survival than class-II. The capacity of DSA I to fix C1q did not correlate with rejection, graft function or graft loss.ConclusionsC1q testing in pretransplant sera with DSA was unable to predict acute antibody-mediated rejection or early graft loss, but the presence of DSA class I compared to DSA only class II did. Despite non-fixing complement in vitro, pretransplant C1q-negative DSA I can mediate rejection and graft loss.© 2013.

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