• Dimas Ikeoka, Christoph Pachler, Stefan Korsatko, Julia K Mader, Heinz Weinhandl, Manfred Bodenlenz, Johannes Plank, Karl-Heinz Smolle, Martin Ellmerer, and Thomas R Pieber.
• Division of Endocrinology and Nuclear Medicine, Department of Internal Medicine, Medical University Graz, Auenbruggerplatz 15, A-8010 Graz, Austria. dimas.ikeoka@healthsite.at
• Cytokine. 2010 Jun 1; 50 (3): 284-91.

AbstractCytokines are inflammatory mediators of major relevance during sepsis. Recent evidence shows that adipose tissue can produce many distinct cytokines under physiological and pathological conditions, but the role of cytokines produced in adipose tissue was not addressed in sepsis. In the present study the open-flow microperfusion (OFM) technique was used to investigate whether the cytokines produced in subcutaneous adipose tissue (SAT) of patients with severe sepsis correlate with clinical variables. Interstitial fluid effluent samples were collected using an OFM catheter inserted in the abdominal SAT of nine patients with severe sepsis. Blood samples were withdrawn concomitantly and interleukin-1beta (IL-1beta), IL-8, IL-6 and tumor necrosis factor alpha (TNF-alpha) were measured both in SAT effluent and serum samples. Different time profiles were registered for each cytokine. IL-1beta increased in a time-dependent manner, indicating a localized response against the catheter insertion. Interleukin-1beta, 6 and 8 were higher in SAT than in serum suggesting they were locally produced. Diastolic blood pressure (DBP) negatively correlated with IL-1beta, IL-6 and IL-8 in SAT indicating a possible interaction between adipose tissue inflammation and vascular tone regulation. A multiple regression analysis disclosed that mean DBP was significantly related to IL-6 concentrations in SAT (B=-43.9; R-square=0.82; P=0.002).Copyright 2010 Elsevier Ltd. All rights reserved.

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