• J. Clin. Microbiol. · Mar 2013

    Multiplex real-time PCR assay for detection of methicillin-resistant Staphylococcus aureus (MRSA) strains suitable in regions of high MRSA endemicity.

    • Jeong-Uk Kim, Choong-Hwan Cha, Hae-Kyong An, Ho-Jun Lee, and Mi-Na Kim.
    • Department of Laboratory Medicine, Gangneung Asan Hospital, University of Ulsan College of Medicine, Gangneung, South Korea. jukim@gnah.co.kr
    • J. Clin. Microbiol. 2013 Mar 1; 51 (3): 1008-13.

    AbstractA multiplex real-time PCR assay that simultaneously detects the mecA, staphylococcal cassette chromosome (SCCmec)-open reading frame X (orfX) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistant Staphylococcus aureus (MRSA) infections.

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