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J. Clin. Microbiol. · Jun 2013
Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection.
- Karsten Becker, Anders R Larsen, Robert L Skov, Gavin K Paterson, Mark A Holmes, Artur J Sabat, Alexander W Friedrich, Robin Köck, Georg Peters, and André Kriegeskorte.
- Institute of Medical Microbiology, University Hospital Münster, Münster, Germany. kbecker@uni-muenster.de
- J. Clin. Microbiol. 2013 Jun 1; 51 (6): 1917-9.
AbstractA mecC (mecALGA251)-adapted multiplex PCR-based methicillin-resistant Staphylococcus aureus (MRSA) detection assay was evaluated using an international, spa-typed Staphylococcus aureus collection comprising 51 mecC-positive MRSA, 240 mecA-positive MRSA, and 50 mecA- and mecC-negative methicillin-susceptible S. aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection.
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