• Naoki Wada, Mona A A Zaki, Yumiko Hori, Koji Hashimoto, Machiko Tsukaguchi, Yoichi Tatsumi, Jun Ishikawa, Nobuhiko Tominaga, Hiroto Sakoda, Hironori Take, Mitsuru Tsudo, Maki Kuwayama, Eiichi Morii, Katsuyuki Aozasa, and Osaka Lymphoma Study Group.
• Department of Pathology, Osaka University Graduate School of Medicine, Suita, Osaka, Japan.
• Histopathology. 2012 Jan 1; 60 (2): 313-9.

AimsTo evaluate the role of tumour-associated macrophages (TAMs) of the M1 and M2 types in the behaviour of diffuse large B-cell lymphoma (DLBCL).Methods And ResultsDouble immunohistochemical staining of HLA-DR/CD68 (M1) or CD163/CD68 (M2) was performed in 101 cases of DLBCL. CD68+ cells represent the total number of TAMs. The average number of double-positive cells was counted, and the cut-off value was set at the mean number of counts, i.e. 30.7 and 27.0 for M1 TAMs and M2 TAMs, respectively. That for total TAMs was set at the 90th percentile number of total counts, i.e. 132.3. Cases were categorized into three pairs: high (34 cases) and low (67 cases) M1 TAM groups, high (39 cases) and low (62 cases) M2 TAM groups, and high (10 cases) and low (91 cases) total TAM groups. The difference in overall survival rates was statistically significant between the high and low M2 TAM groups (P < 0.01) and between the high and low total TAM groups (P < 0.05). Multivariate analysis revealed that the presence of a bulky mass and a higher number of M2 TAMs were significant factors for poor prognosis (P < 0.05).ConclusionsEstimation of specific type of macrophages, of the M1 and M2 types, is superior to the estimation of TAMs as a whole (CD68+ cells) for prediction of the prognosis of DLBCL patients.© 2011 Blackwell Publishing Limited.

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