• J Shoulder Elbow Surg · Mar 2013

    Tenocytes of chronic rotator cuff tendon tears can be stimulated by platelet-released growth factors.

    • Sven Hoppe, Mauro Alini, Lorin M Benneker, Stefan Milz, Pascal Boileau, and Matthias A Zumstein.
    • Orthopedic Sports Medicine, Department of Orthopedic Surgery and Traumatology, University of Bern, Inselspital, CH- 3010 Bern, Switzerland.
    • J Shoulder Elbow Surg. 2013 Mar 1; 22 (3): 340-9.

    BackgroundBone-to-tendon healing after rotator cuff repairs is mainly impaired by poor tissue quality. The tenocytes of chronic rotator cuff tendon tears are not able to synthesize normal fibrocartilaginous extracellular matrix (ECM). We hypothesized that in the presence of platelet-released growth factors (PRGF), tenocytes from chronically retracted rotator cuff tendons proliferate and synthesize the appropriate ECM proteins.Materials And MethodsTenocytes from 8 patients with chronic rotator cuff tears were cultured for 4 weeks in 2 different media: standard medium (Iscove's Modified Dulbecco's Media + 10% fetal calf serum + 1% nonessential amino acids + 0.5 μg/mL ascorbic acid) and media with an additional 10% PRGF. Cell proliferation was assessed at 7, 14, 21, and 28 days. Messenger (m)RNA levels of collagens I, II, and X, decorin, biglycan, and aggrecan were analyzed using real time reverse-transcription polymerase chain reaction. Immunocytochemistry was also performed.ResultsThe proliferation rate of tenocytes was significantly higher at all time points when cultured with PRGF. At 21 days, the mRNA levels for collagens I, II, and X, decorin, aggrecan, and biglycan were significantly higher in the PRGF group. The mRNA data were confirmed at protein level by immunocytochemistry.ConclusionsPRGFs enhance tenocyte proliferation in vitro and promote synthesis of ECM to levels similar to those found with insertion of the normal human rotator cuffs.Clinical RelevanceBiologic augmentation of repaired rotator cuffs with PRGF may enhance the properties of the repair tissue. However, further studies are needed to determine if application of PRGF remains safe and effective in long-term clinical studies.Level Of EvidenceBasic Science Study, Cell Biology.Copyright © 2013 Journal of Shoulder and Elbow Surgery Board of Trustees. Published by Mosby, Inc. All rights reserved.

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