• Malika A Benahmed, Karim Elbayed, François Daubeuf, Nicola Santelmo, Nelly Frossard, and Izzie J Namer.
• Faculté de Médecine, Laboratoire d'Imagerie et de Neurosciences Cognitives (LINC), Institut de Physique Biologique, Université de Strasbourg/CNRS UMR7237, Strasbourg Cedex, France; Laboratoire de RMN et Biophysique des membranes, Institut de Chimie, Université de Strasbourg/CNRS UMR7177, Strasbourg, France.
• Magn Reson Med. 2014 Jan 1; 71 (1): 35-43.

PurposeUsing the metabolomics by NMR high-resolution magic angle spinning spectroscopy, we assessed the lung metabolome of various animal species in order to identify the animal model that could be substituted to human lung in studies on fresh lung biopsies.MethodsThe experiments were conducted on intact lung biopsy samples of pig, rat, mouse, and human using a Bruker Advance III 500 spectrometer. Thirty-five to 39 metabolites were identified and 23 metabolites were quantified. Principal component analysis, partial least-squares discriminant analysis, and analysis of variance tests were performed in order to compare the metabolic profiles of each animal lung biopsies to those of the human lung.ResultsThe metabolic composition between human and pig lung was similar. However, human lung was distinguishable from mouse and rat regarding: Trimethylamine N-oxide and betaïne which were present in rodents but not in human lung, carnitine, and glycerophosphocholine which were present in mouse but not in human lung. Conversely, succinic acid was undetected in rat lung. Furthermore, fatty acids concentration was significantly higher in rodent lungs compared to human lung.ConclusionUsing the metabolomics by NMR high-resolution magic angle spinning spectroscopy on lung biopsy, samples allowed to highlight that pig lung seems to be close to human lung as regarding its metabolite composition with more similarities than dissimilarities.Copyright © 2013 Wiley Periodicals, Inc.

29 keywords...

hide…