• Behrooz Ilkhanizadeh, Leila Zarei, Negin Farhad, Mehran Bahrami-Bukani, and Rahim Mohammadi.
• Department of Pathology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
• Injury. 2017 Jul 1; 48 (7): 1480-1485.

BackgroundEmployment of regenerative properties of cells at the service of nerve repair has been initiated during recent decades. Effects of local transplantation of bone marrow-derived mast cells on peripheral nerve regeneration were studied using a rat sciatic nerve transection model.Materials And MethodsA 10-mm sciatic nerve defect was bridged using a conduit chitosan-based hybrid conduit filled with BMMCs in BMMC group. In positive control group (Pos), the conduit was filled with phosphate-buffered saline alone. The regenerated nerve fibers were studied within 12 weeks after surgery. In sham-operated group, the sciatic nerve was only exposed and manipulated. In negative control (Neg) a 10-mm sciatic nerve defect was created and the nerve stumps were sutured to the adjacent muscles. The regenerated nerve fibers were studied functionally, biomechanically, histologically and immunohiscochemically.ResultsFunctional and biomechanical studies confirmed faster recovery of regenerated axons in BMMCs transplanted animals compared to Pos group (p<0.05). Morphometric indices of the regenerated fibers showed that the number and diameter of the myelinated fibers were significantly higher in BMMCs transplanted animals than in Pos group (p<0.05). In immunohistochemistry, location of reactions to S-100 in BMMCs transplanted animals was clearly more positive than that in Pos group.ConclusionsBMMCs transplantation could be considered as a readily accessible source of cells that could improve functional recovery of transected sciatic nerve.Copyright © 2017 Elsevier Ltd. All rights reserved.

22 keywords...

hide…