• Pharmacol Rep · Aug 2017

    Anti-inflammatory and anti-nociceptive effects of strontium ranelate on the zymosan-induced temporomandibular joint inflammatory hypernociception in rats depend on TNF-α inhibition.

    • Sheila Moreira Alves, Susana Capistrano Abreu, Jonas Cavalcante Lemos, Gomes Francisco Isaac Fernandes FIF Federal University of Ceará, Avenida Comandante Maurocélio Rocha Pontes, 100 Derby, CEP: 62.042-280, Sobral, Ceará, Brazil. Electronic, Shirley Moreira Alves, Danielle Rocha do Val, Raul Sousa Freitas, Pereira Karuza Maria Alves KMA Federal University of Ceará, Avenida Comandante Maurocélio Rocha Pontes, 100 Derby, CEP: 62.042-280, Sobral, Ceará, Brazil. Electronic addr, Vicente de Paulo Teixeira Pinto, de Castro Brito Gerly Anne GA Department of Morphology, Federal University of Ceará, Rua Delmiro de Farias, Porangabussu, CEP: 60440-261, Fortaleza, Ceará, Brazil. Electr, Mirna Marques Bezerra, Gerardo Cristino-Filho, and Hellíada Vasconcelos Chaves.
    • Federal University of Ceará, Avenida Comandante Maurocélio Rocha Pontes, 100 Derby, CEP: 62.042-280, Sobral, Ceará, Brazil. Electronic address: eishyla2010@hotmail.com.
    • Pharmacol Rep. 2017 Aug 1; 69 (4): 764-772.

    BackgroundTemporomandibular joint (TMJ) disorders show inflammatory components, heavily impacting on quality of life. Strontium ranelate has previously shown anti-inflammatory and antinociceptive effects on other experimental inflammatory pain models. Thus, we aim to investigate the strontium ranelate efficacy in reducing the zymosan-induced inflammatory hypernociception in the TMJ of rats by evaluating the TNF-α, IL-1β, and hemeoxygenase-1 (HO-1) involvement.MethodsWistar rats were treated with strontium ranelate (0.5, 5 or 50 mg/kg, per os) 1 h before zymosan injection (iart). Mechanical threshold was assessed by Von Frey test and synovial lavage was collected for leukocyte counting and myeloperoxidase measurement, joint tissue and trigeminal ganglion were excised for histopathological analysis (H&E) and TNF-α/IL-1β levels dosage (ELISA). Moreover, rats were pre-treated with ZnPP-IX (3 mg/kg, sc), a specific HO-1 inhibitor, before strontium ranelate administration (0.5 mg/kg, per os), and Evans Blue (5 mg/kg, iv) was administered to assess plasma extravasation. Pre-treatment with indomethacin (5 mg/kg, sc) was used as positive control while the sham group received 0.9% sterile saline (per os and iart).ResultsStrontium ranelate did not reduce leukocyte counting, myeloperoxidase activity, Evans Blue extravasation, IL-1β levels, and TNF-α/IL-1β immunolabeling; but it increased the nociceptive threshold and reduced TNF-α levels. Additionally, HO-1 inhibition did not change the strontium ranelate effects.ConclusionStrontium ranelate may achieve its antinociceptive effects through the reduction of TNF-α levels in the trigeminal ganglion, but not suppressing IL-1β expression nor inducing the HO-1 pathway.Copyright © 2017 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.

      Pubmed     Full text   Copy Citation     Plaintext  

      Add institutional full text...

    Notes

     
    Knowledge, pearl, summary or comment to share?
    300 characters remaining
    help        
    You can also include formatting, links, images and footnotes in your notes
    • Simple formatting can be added to notes, such as *italics*, _underline_ or **bold**.
    • Superscript can be denoted by <sup>text</sup> and subscript <sub>text</sub>.
    • Numbered or bulleted lists can be created using either numbered lines 1. 2. 3., hyphens - or asterisks *.
    • Links can be included with: [my link to pubmed](http://pubmed.com)
    • Images can be included with: ![alt text](https://bestmedicaljournal.com/study_graph.jpg "Image Title Text")
    • For footnotes use [^1](This is a footnote.) inline.
    • Or use an inline reference [^1] to refer to a longer footnote elseweher in the document [^1]: This is a long footnote..

    hide…