• Mol. Cell. Probes · Dec 2015

    Real-time reverse transcriptase polymerase chain reaction assays for Middle East Respiratory Syndrome.

    • Christina E Douglas, David A Kulesh, James G Jaissle, and Timothy D Minogue.
    • Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Disease, Fort Detrick, Maryland, USA.
    • Mol. Cell. Probes. 2015 Dec 1; 29 (6): 511-513.

    AbstractHere we designed and tested two highly specific quantitative TaqMan(®)-MGB-based reverse transcriptase quantitative polymerase chain reaction (RT-qPCR) assays for Middle East Respiratory Syndrome (MERS). The primers and probes for these assays were evaluated and found to have a limit of detection (LOD) of 0.005 plaque-forming units/PCR (pfu/PCR).Published by Elsevier Ltd.

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