• Burns · Mar 2018

    Comparative Study

    Histological observation of hairless mice skin after exposure to Simulated Solar Light: Comparison between the histological findings with different methodologies and 3R principle correlations.

    • Bryan Hudson Hossy, Alvaro Augusto da Costa Leitão, Renata Bosco Torres, Marcia Ramos-E-Silva, Nádia Campos de Oliveira Miguel, and Marcelo de Pádula.
    • Programa de Pós Graduação em Clínica Médica, Faculdade de Medicina - Serviço de Dermatologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
    • Burns. 2018 Mar 1; 44 (2): 359-369.

    BackgroundAlbino hairless mouse (AHM) has been used as a biological model in photodermatology. However, the experimental landscape is diverse to follow and need particular attention.PurposeIrradiation parameters were investigated for the development of a protocol to assess alterations in the AHM skin using Simulated Solar Light (SSL). The present study was compared with published articles (last 15 years) according to irradiation protocols, morphological findings to minimize animal suffering and UV exposure.Materials And MethodsThree groups: Control (G1), experimental - sunburn (G2) and skin photodamage assay (G3). G2 were immobilized and exposed to SSL once for 15, 30 and 45min. G3 were exposed to SSL, without immobilization, for 15min once a day for one week. The dorsal skin was analyzed using hematoxylin and eosin technique.ResultsG2 displayed different sunburn degrees. Based on the profile of the observed morphological alterations, a 15min irradiation was chosen as the exposure time to expose G3, without immobilization, for 5 consecutive days.ConclusionThese conditions produced the same morphological changes in the AHM with a shorter solar exposure time, without immobilizing the animals but using environmental exposure fluences, conforming to 3R (reduction - refinement - replacement) recommendations.Copyright © 2017 Elsevier Ltd and ISBI. All rights reserved.

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