• Marius I Ilie, Sandra Lassalle, Elodie Long-Mira, Christelle Bonnetaud, Olivier Bordone, Virginie Lespinet, Aude Lamy, Jean-Christophe Sabourin, Juliette Haudebourg, Catherine Butori, Nicolas Guevara, Isabelle Peyrottes, Jean-Louis Sadoul, Alexandre Bozec, José Santini, David Capper, Andreas von Deimling, Jean-François Emile, Véronique Hofman, and Paul Hofman.
• 1 Team 3, Faculty of Medicine, French Institute of Health and Medical Research (INSERM) U1081, French National Center for Scientific Research (CNRS) UMR 7284, Institute for Research on Cancer and Aging, Nice, France .
• Thyroid. 2014 May 1; 24 (5): 858-66.

BackgroundThe aim of this study was to compare the detection of BRAF(V600E) by immunohistochemistry (IHC) using a mutation-specific antibody with molecular biology methods for evaluation of papillary thyroid carcinoma (PTC) patients.Patients And MethodsThis study concerned 198 consecutive conventional PTC patients, of which the majority were women (133/198; 67%), with a mean age of 56 years (range 19-79 years). BRAF mutation analysis was performed using DNA-based (direct sequencing, pyrosequencing, and SNaPshot) and IHC (VE1 antibody) methods. The sensitivity and specificity of IHC for BRAF(V600E) was compared with the molecular biology data.ResultsA BRAF mutational result was obtained in 194 cases. A BRAF(V600E) mutation was detected in 153/194 (79%) cases of PTC when using at least one molecular method, and in 151/194 (78%) cases with IHC. No false positive results were noted using IHC to detect the BRAF(V600E) mutation. Besides this mutation, other rare BRAF mutations (BRAF(V600K) and BRAF(K601E)), used as negative controls, were consistently negative with IHC. The sensitivity and specificity of IHC for the detection of this mutation were 98.7% and 100% respectively. The IHC test demonstrated excellent performance at a level equivalent to two DNA-based counterparts (pyrosequencing and SNaPshot). Failure to achieve a result was more frequent with the direct sequencing method than with the three other methods.ConclusionIHC using the VE1 antibody is a specific and sensitive method for the detection of the BRAF(V600E) mutation in PTC. IHC may be an alternative to molecular biology approaches for the routine detection of this mutation in PTC patients.

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