• Spine · Nov 2018

    Ascorbic Acid Attenuates Multifidus Muscles Injury and Atrophy After Posterior Lumbar Spine Surgery by Suppressing Inflammation and Oxidative Stress in a Rat Model.

    • Pan Tang, Ren Zhu, Yu Gu, Jia-Ming Gu, Zi-Ang Xie, Jia-Qi Xu, Xiang-De Zhao, Kang-Mao Huang, Ji-Ying Wang, Xue-Sheng Jiang, Shun-Wu Fan, and Zhi-Jun Hu.
    • Department of Orthopaedics, Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, China.
    • Spine. 2018 Nov 1; 43 (21): E1249-E1259.

    Study DesignA rat model of multifidus muscles injury and atrophy after posterior lumbar spine surgery.ObjectiveWe determined the effect of ascorbic acid (AA) on the postoperative multifidus muscles in rat model.Summary Of Background DataPrevious studies show oxidative stress and inflammation are two main molecular mechanisms in multifidus muscle injury and atrophy after posterior lumbar surgery. AA may have a protective effect in postoperative multifidus muscles.MethodsRats were divided into sham surgery, control surgery, and surgery plus AA groups. Multifidus muscles of the control and AA groups were excised from the osseous structures. The muscles were retracted continuously for 2 hours. In the sham and AA groups, AA was administered via oral gavage daily in the first week. In each group, the oxidative stress was evaluated by measuring malondialdehyde (MDA) and Total superoxide dismutase (T-SOD). The inflammation, fat degeneration, or fibrosis of multifidus muscle were evaluated by quantitative real-time polymerase chain reaction (q-PCR), histology, or immunohistochemical analysis.ResultsT-SOD activity was significantly lower in the control group than that in the AA group in the first week. MDA levels were significantly higher in the AA group. Interleukin-6 and tumor necrosis factor-α in multifidus muscles also showed significant differences when treated with AA. The inflammation score on histology was significantly lower in the AA group postoperatively in the first week. In the long run, marker genes for fibrosis and fat degeneration, and fibrosis and fat degeneration scores, were significantly lower in the AA than the control group on days 14 and 28 postoperatively.ConclusionIn conclusion, AA attenuated the oxidative stress and inflammation response in the postoperative multifidus muscles, and remarkable differences were observed from the histological assessment and related marker genes expression. Our results provided important insight into the anti-inflammatory and anti-oxidative effects of AA in the postoperative multifidus muscles.Level Of EvidenceN/A.

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