• Chunsheng Hu, Zhenzhen Cai, Yuxin Lu, Xiaochen Cheng, Zuze Wu, and Qinglin Zhang.
• College of Life Science and Bio-engineering, Beijing University of Technology, Beijing 100124, People's Republic of China; Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, People's Republic of China.
• Neurosci. Lett. 2016 Oct 6; 632: 157-62.

AbstractWe investigated the antinociceptive effect of local intramuscular injection of a plasmid encoding human proenkephalin (pVAX1-hPPE) on postoperative pain in rats. Male Sprague-Dawley rats with incision-induced pain were intramuscularly injected into injured plantaris muscle with empty vector (pVAX1) or pVAX1-hPPE, respectively. Paw mechanical threshold and thermal latency in the 200μg pVAX1-hPPE treated rats were significantly higher at 6h and on 1day, and lasted until day 7 after intramuscular administration, respectively. The analgesic effects were reversed by methylnaltrexone, suggesting that the antinociceptive effect of pVAX1-hPPE was mediated through peripheral opioid receptor pathway. In contrast, incisional or pVAX1-treated rats did not significantly affect pain thresholds. These results demonstrated that single intramuscular injection of pVAX1-hPPE attenuated incision-induced pain in rats, and it is worthy of further study as a potential gene therapy for postoperative pain. Copyright © 2016 Elsevier Ireland Ltd. All rights reserved.

23 keywords...

hide…