• Adam J Singer, Jimmy Toussaint, Won Taek Chung, Steve A McClain, Clark Richard A F RAF Department of Dermatology, Stony Brook University, Stony Brook, NY, United States; Department of Biomedical Engineering, Stony Brook University, Sto, Eilon Asculai, Dafna Geblinger, and Lior Rosenberg.
• Department of Emergency Medicine, Stony Brook University, Stony Brook, NY, United States. Electronic address: adam.singer@stonybrook.edu.
• Burns. 2018 Jun 1; 44 (4): 896-904.

ObjectivesThere are no well accepted animal models of chronic wounds, limiting advances in understanding and treatment of chronic ulcers. We developed a porcine wound model which combines multiple factors involved in chronic wounds to create a contaminated necrotic eschar and evaluated the debriding efficacy of a novel bromelain based enzymatic debriding agent (EscharEx).MethodsContaminated ischemic wounds were created on the flanks of domestic pigs by 'sandwiching' the skin between 2 'O' rings (1 placed on the surface of the skin and the other underneath the skin) for 24h prior to dermatomal excision of the necrotic eschar and its contamination with Staphylococcus aureus and Candida albicans. After confirming the development of infected eschars, additional animals were used to compare the effects of daily application of topical EscharEx or its hydrating vehicle on eschar debridement as a control.ResultsIn all cases, application of the 'O' rings resulted in full thickness necrotic ecshars with invasive infections, which did not reepithelialize and sloughed off spontaneously within 14-21 days. All wounds reepithelialized within 28-42 days forming contracted scars. All EscharEx treated eschars were completely debrided within 7-9 days, while no debridement was evident in eschars treated with the control gel.ConclusionsOur model simulates the initial phase of chronic wounds characterized by a contaminated necrotic eschar allowing evaluation of wound debriding agents, and that a bromelain-based debriding agent completely debrides the contaminated necrotic eschars within one week in this model.Copyright © 2017 Elsevier Ltd and ISBI. All rights reserved.

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