• Acta histochemica · Jan 2017

    Identification of AgNORs and cytopathological changes in oral lichen planus lesions.

    • Stefânia Jeronimo Ferreira, Maria Ângela Naval Machado, de Lima Antônio Adilson Soares AA School of Dentistry, Federal University of Paraná, Curitiba, Pr, Brazil. Electronic address: antollima@hotmail.com., Johann Aline Cristina Batista Rodrigues AC Graduate Program in Dentistry, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conc, Ana Maria Trindade Grégio, and Luciana Reis Azevedo-Alanis.
    • Graduate Program in Dentistry, School of Health and Biosciences, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição, 1155, Curitiba, Pr 80215-901, Brazil. Electronic address: s.j.f_1985@hotmail.com.
    • Acta Histochem. 2017 Jan 1; 119 (1): 32-38.

    ObjectiveTo evaluate cytopathological changes in epithelial cells of the oral mucosa of patients with oral lichen planus (OLP) compared with patients without OLP.Subjects And MethodsSwabs were collected from the oral mucosa of 20 patients with OLP (case group) and 20 patients without OLP (control group) using liquid-based cytology. After Papanicolaou staining, the smears were characterized based on Papanicolaou classification and degree of maturation. Nuclear area (NA) measurements, cytoplasmic area (CA) measurements, and the NA/CA ratio were determined from 50 epithelial cells per slide. For quantification of argyrophilic nucleolar organizer regions (AgNORs), the smears were stained with silver nitrate, and the number of AgNORs was counted in 100 cells.ResultsIn both groups, there was a predominance of Papanicolaou Class I nucleated cells in the superficial layer. The average values of NA (p>0.05) and CA (p=0.000) were greater in the case group (NA=521.6, CA=22,750.3) compared with the control group (NA=518.9, CA=18,348.0). The NA/CA ratio was 0.025 for the case group and 0.031 for the control group (p=0.004). There was no significant difference between the mean AgNORs values of both groups (p>0.05).ConclusionThe oral mucosa of patients with OLP exhibited significant cytomorphometric changes. However, there was no evidence of malignancy.Copyright © 2016 Elsevier GmbH. All rights reserved.

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