• Neuroscience letters · Jul 1997

    Destruction of neurokinin-1 receptor expressing cells in vitro and in vivo using substance P-saporin in rats.

    • R G Wiley and D A Lappi.
    • Neurology Service, VAMC, Nashville, TN 37212-2637, USA. wileyrg@ctrvax.vanderbilt.edu
    • Neurosci. Lett. 1997 Jul 18; 230 (2): 97-100.

    AbstractSubstance P (SP) acts on neurons through the neurokinin-1 (NK-1) receptor. Conjugation of SP to the ribosome inactivating protein, saporin (SAP), produces a cytotoxin selective for cells that express the NK-1 receptor. SP-SAP cytotoxicity was inhibited by pre-treating the toxin to reduce the disulfide bond connecting SP to SAP or by pre-incubation with anti-SP antiserum or by SP analog showing that SP-SAP acts through binding of the SP moiety to NK-1 receptors. Injection of SP-SAP into the striatum selectively destroyed NK-1 receptor expressing interneurons. These results show that SP-SAP will be useful for studying the function of NK-1 receptor expressing neurons.

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