• J. Clin. Microbiol. · Oct 1996

    Rapid identification of Toscana virus by nested PCR during an outbreak in the Siena area of Italy.

    • M Valassina, M G Cusi, and P E Valensin.
    • Department of Molecular Biology, University of Siena, Italy.
    • J. Clin. Microbiol. 1996 Oct 1; 34 (10): 2500-2.

    AbstractThe sand fly-transmitted Toscana virus is recognized as an etiologic agent of an aseptic meningitis with a long convalescence. This infection has been reported overall in many tourists or in a seronegative population circulating in endemic Mediterranean areas (Italy, Portugal, Egypt, and Cyprus). We report a cluster of acute Toscana virus infections in the local population during the summer of 1995. Twenty-one clinical cases of meningitis were investigated for the presence of Toscana virus by nested PCR performed on the S segment of the virus RNA extracted from cerebrospinal fluid samples.

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