• Diagn. Microbiol. Infect. Dis. · Oct 2015

    Case Reports

    Unbiased metagenomic sequencing complements specific routine diagnostic methods and increases chances to detect rare viral strains.

    • Dagmara W Lewandowska, Osvaldo Zagordi, Andrea Zbinden, Macé M Schuurmans, Peter Schreiber, Fabienne-Desirée Geissberger, Jon B Huder, Jürg Böni, Christian Benden, Nicolas J Mueller, Alexandra Trkola, and Michael Huber.
    • Institute of Medical Virology, University of Zurich, Winterthurerstrasse 190, 8057 Zurich, Switzerland.
    • Diagn. Microbiol. Infect. Dis. 2015 Oct 1; 83 (2): 133-8.

    AbstractMultiplex PCR assays for respiratory viruses are widely used in routine diagnostics, as they are highly sensitive, rapid, and cost effective. However, depending on the assay system, cross-reactivity between viruses that share a high sequence homology as well as detection of rare virus isolates with sequence variations can be problematic. Virus sequence-independent metagenomic high-throughput sequencing allows for accurate detection of all virus species in a given sample, as we demonstrate here for human Enterovirus and Rhinovirus in a lung transplant patient. While early in infection a commercial PCR assay recorded Rhinovirus, high-throughput sequencing correctly identified human Enterovirus C104 as the source of infection, highlighting the potential of the technology and the benefit of applying open assay formats in complex diagnostic situations. Copyright © 2015 Elsevier Inc. All rights reserved.

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