• Lin Yang, Liang Guo, Junmei Xu, Yaping Wang, Dingquan Zou, and Xinghua Jiang.
• Department of Anesthesiology, Second Xiangya Hospital, Central South University, Changsha 410011, China.
• Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2017 May 28; 42 (5): 511-516.

ObjectiveTo detect the effect of pulse radiofrequency (PRF) treatment on the neuropathic pain established by L5-spinal nerve ligation (SNL) on rats, and to investigate if PRF treatment would affect the expression of autophagy related protein LC3 and autophagy related receptor P62 at the dorsal horn.
 Methods: A total of 36 male Sprague-Dawley rats were randomly divided into 3 groups: a Sham group, a SNL group, and a SNL+PRF group. The 50% paw withdrawal mechanical threshold (PWMT) was detected at 1 day before and 1, 3, 7, 14 and 28 days post-operation by using Von-Frey filaments. The autophagy related protein LC3 and autophagy related receptor P62 were investigated by Western blot.
 Results: Compared with the Sham group, the PWMT significantly decreased in the SNL group at each time points (P<0.05); in SNL+PRF group, PRF treatment could elevate the PWMT at the 1st day post-operation and lasted for 28 days (P<0.05). What's more, SNL could elevate the LC3-II and P62 levels at the 7th day post-operation (P<0.05), which were decreased by the PRF treatment (P<0.05).
 Conclusion: PRF treatment could improve SNL-induced the neuropathic pain, which might be partly due to the regulatory effects on the autophagy levels at the spinal dorsal horn.

17 keywords...

hide…