• Rummenigge Oliveira Silva, Bruna Lopes da Costa, Carolina Nunes da Silva, Thaís Maria da Mata Martins, Lays Fernanda Nunes Dourado, Alfredo Miranda de Goes, Miriam Teresa Lopes, Carlos Edmundo Salas, Armando da Silva-Cunha, and Flavia Rodrigues da Silva.
• Faculdade de Farmácia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.
• Burns. 2020 Jun 1; 46 (4): 928-936.

IntroductionChemical ocular burns are among the most frequently eye-related injuries, which require immediate and intensive evaluation and care since they may lead to potential complications such as superinfection, corneal perforation, and blindness.Vasconcellea cundinamarcensis, a species from Caricaceae family, contains highly active proteolytic enzymes in its latex that show healing activity in animal models bearing lesions of different etiologies.MethodsWe evaluate the ocular toxicity of the proteolytic fraction from V. cundinamarcensis (P1G10) by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and Hen's Egg Test-Chorioallantoic Membrane test. The corneal healing property of P1G10 was studied by the ethanol-chemical burn in the rabbit's eyes.ResultsP1G10 is safe for ocular administration, except when administrated at 10μg/mL. P1G10 at 1μg/mL accelerates the corneal re-epithelization achieving complete wound closure after 72h of chemical burn. Also, P1G10 modulated the inflammatory response and controlled the arrangement of collagen fibers in the stroma, demonstrating its potential corneal healing properties.ConclusionsOur work was the first one to evaluate the ophthalmic application of P1G10. Here we demonstrated that P1G10 is suitable for ocular administration and it has a promising corneal healing activity which may emerge as a new pharmacological tool to the development of a new drug for ocular surface chemical injuries in the future.Copyright © 2019 Elsevier Ltd and ISBI. All rights reserved.

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