• Ana Carolina Guidi, Mariana Nascimento de Paula, Mirela Mosela, Leonardo Alan Delanora, Gustavo Cesar Abreu Soares, Gutierrez Rodrigues de Morais, Daniela Cristina de Medeiros, Admilton Gonçalves de Oliveira Junior, Cláudio Roberto Novello, Mauro Luciano Baesso, Eneri Vieira de Souza Leite-Mello, and João Carlos Palazzo de Mello.
• Department of Pharmacy, Laboratory of Pharmaceutical Biology - Palafito, Universidade Estadual de Maringá, BR-87020-900, Maringá, Brazil.
• Injury. 2020 Apr 1; 51 (4): 840-849.

AbstractPoincianella pluviosa has already been described as capable of healing skin wounds. In an attempt to prolong contact of the drug with the wound, it was proposed in this study to evaluate wound healing using a crude extract (CE) of P. pluviosa incorporated in carboxymethylcellulose polymer films. The chromatographic profile of the semipurified fraction of P. pluviosa was evaluated by ultra-high performance liquid chromatography (UHPLC), confirming the compounds gallic acid, geraniin, and ellagic acid. The films were evaluated for their physical and mechanical properties, water vapor permeability, moisture absorption capacity, and FTIR spectroscopy. For in vivo experiments, wounds were made on the back of rats and treated daily for 4, 7, 10, or 14 days with film containing CE or control film. At the end of each period, skin permeation analysis and histological analysis were made using re-epithelialisation, cell proliferation, and collagen formation. Statistical significance was determined by GraphPad Prism using t test and Mann-Whitney test. Anti-staphylococcal activity was evaluated with standard strains of Staphylococcus aureus, methicillin-resistant, and coagulase negative. It was demonstrated that the presence of CE in the films increased the capacity to absorb water and decreased resistance and permeability. The CE of the film permeated the skin, reaching the dermis and was able to influence re-epithelisation, cell proliferation, and collagen formation. Satisfactory results were observed against S. aureus strains, particularly coagulase negative. Films with CE of P. pluviosa can be an alternative in the wound healing, protecting against opportunistic infections and giving comfort to the patient.Copyright © 2020 Elsevier Ltd. All rights reserved.

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