• Michaela A Gazdik, Jana Coombs, John P Burke, and Bert K Lopansri.
• Department of Clinical Epidemiology and Infectious Diseases, Intermountain Medical Center, Murray, Utah, USA michaela.gazdik@imail.org.
• J. Clin. Microbiol. 2016 Feb 1; 54 (2): 312-6.

AbstractRecent outbreaks of carbapenem-resistant Enterobacteriaceae infections associated with duodenoscopes used for endoscopic retrograde cholangiopancreatography have highlighted the challenge of cleaning and high-level disinfection of these instruments. The Food and Drug Administration has suggested that duodenoscope surveillance by microbiological culturing, along with strict adherence to reprocessing protocols, may help reduce the risk of duodenoscope-associated infection transmission. We developed and validated an effective, user-friendly duodenoscope sampling and culture protocol and compared its performance to the interim Centers for Disease Control and Prevention-recommended guidelines. Our protocol resulted in a 65% recovery rate for Gram-negative organisms, demonstrating a 2-fold increased recovery rate compared to the CDC method. The implementation of this protocol may increase the feasibility of duodenoscope surveillance for microbiology laboratories and endoscopy departments. Copyright © 2016, American Society for Microbiology. All Rights Reserved.

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