• Naoya Yamada, Tadayoshi Karasawa, Taiichi Wakiya, Ai Sadatomo, Homare Ito, Ryo Kamata, Sachiko Watanabe, Takanori Komada, Hiroaki Kimura, Yukihiro Sanada, Yasunaru Sakuma, Koichi Mizuta, Nobuhiko Ohno, Naohiro Sata, and Masafumi Takahashi.
• Division of Inflammation Research, Center for Molecular Medicine, Jichi Medical University, Tochigi, Japan.
• Am. J. Transplant. 2020 Jun 1; 20 (6): 1606-1618.

AbstractHepatic ischemia-reperfusion (I/R) injury is a major problem in liver transplantation (LT). Although hepatocyte cell death is the initial event in hepatic I/R injury, the underlying mechanism remains unclear. In the present study, we retrospectively analyzed the clinical data of 202 pediatric living donor LT and found that a high serum ferritin level, a marker of iron overload, of the donor is an independent risk factor for liver damage after LT. Since ferroptosis has been recently discovered as an iron-dependent cell death that is triggered by a loss of cellular redox homeostasis, we investigated the role of ferroptosis in a murine model of hepatic I/R injury, and found that liver damage, lipid peroxidation, and upregulation of the ferroptosis marker Ptgs2 were induced by I/R, and all of these manifestations were markedly prevented by the ferroptosis-specific inhibitor ferrostatin-1 (Fer-1) or α-tocopherol. Fer-1 also inhibited hepatic I/R-induced inflammatory responses. Furthermore, hepatic I/R injury was attenuated by iron chelation by deferoxamine and exacerbated by iron overload with a high iron diet. These findings demonstrate that iron overload is a novel risk factor for hepatic I/R injury in LT, and ferroptosis contributes to the pathogenesis of hepatic I/R injury.© 2020 The American Society of Transplantation and the American Society of Transplant Surgeons.

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