• Qingxiu Wang, Yupeng Zhao, Min Sun, Sheng Liu, Baolin Li, Lei Zhang, and Longqiu Yang.
• Department of Anesthesiology, East Hospital, Tongji University School of Medicine, 150#, Jimo Road, Shanghai 200120, PR China.
• Toxicol In Vitro. 2014 Oct 1; 28 (7): 1183-9.

ObjectSevoflurane, one of the most commonly used anesthetics in clinic, induced neuroinflammation and caused cognitive impairment. 2-deoxy-d-glucose (2-DG) is a synthetic analogue of glucose and is clinically used in medical imaging safely.MethodsWe examined the effect of 2-DG on sevoflurane-induced neuroinflammation in the mouse primary microglia cells. Mouse microglia cells were treated with 4.1% sevoflurane for 6h to examine the expression of interleukin (IL)-6 and tumor necrosis factor (TNF-α) and activation of nuclear factor-kappa B (NF-κB). Pyrrolidine dithiocarbamate (PDTC) or 2-DG was used 1h before sevoflurane treatment.ResultsIn the present study, we found that sevoflurane increased level of IL-6 and TNF-α through activating NF-κB signaling, and that 2-DG reduced sevoflurane-induced increase in IL-6 and TNF-α and nuclear NF-κB in microglia cells.ConclusionOur data suggests that NF-κB signaling pathway could be a target for sevoflurane-induced neuroinflammation and 2-DG might be a potential therapy to prevent or treat sevoflurane-induced neuroinflammation.Copyright © 2014 Elsevier Ltd. All rights reserved.

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