• Stephanie Antoniol, Nadhira Fidouh, Aiham Ghazali, Houria Ichou, Donia Bouzid, Philippe Kenway, Christophe Choquet, Benoit Visseaux, Enrique Casalino, and Emergency Department study group on respiratory viruses.
• Assistance Publique-Hôpitaux de Paris (AP-HP), Groupe Universitaire Paris Nord Val de Seine, Emergency Department, Paris, France; Université Paris Diderot, Sorbonne Paris Cité, EA 7334, Recherche clinique coordonnée ville-hôpital, Méthodologies et Société (REMES), Paris, France; Study Group for Efficiency and Quality of Emergency Departments and Non-Scheduled Activities Departments, Paris, France.
• J. Clin. Virol. 2018 Feb 1; 99-100: 5-9.

BackgroundNew rapid influenza diagnostic tests (RIDT) are available but their clinical utility in adults has not been validated.ObjectivesTo evaluate the diagnostic performances of OSOM® Ultra Flu A&B a RIDT on viral strains of influenza A/B from the last epidemic season, and its feasibility by Emergency Department (ED) physicians and nurses.Study DesignOf the 1099 patients admitted to the ED with suspected influenza, all having a nasopharyngeal swab tested by the Xpert® Flu PCR and then stored at -20 °C; 500 were selected at random and their samples were tested using the RIDT. Two experts reviewed ED and hospital medical records and all virological data to define influenza cases. Intra- and inter-observer variability were calculated.ResultsOf the 500 patients included 45% were ≥75 years, 122 (24.4%) presented with influenza based on clinical and virological criteria. PCR test performances (%) were Se 98.4 (95% CI 93.6-99.7), Spe 99.7 (98.3-100), PPV 99.2 (94.8-100) and NPV 99.5 (97.9-100); and RIDT performances were Se 95.1 (89.2-97.9), Spe 98.4 (96.4-99.4), PPV 95.1 (87.2-99.9) and NPV 98.4 (96.4-98). There was no difference in test performance between influenza A and B virus nor between the influenza A subtypes. Intra- and inter-observer variability of RIDT were 0.94 (0.89-0.99) and 0.96 (0.92-1).ConclusionOur results show that the Xpert® Flu PCR and the OSOM® Ultra Flu A&B Test perform very well in diagnosing strains of circulating virus in adults and elderly. Our results also confirm the feasibility of this RIDT at point-of-care by ED staff.Copyright © 2017. Published by Elsevier B.V.

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