Journal of tissue engineering and regenerative medicine
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J Tissue Eng Regen Med · Mar 2016
Foreskin-isolated keratinocytes provide successful extemporaneous autologous paediatric skin grafts.
Severe burns in children are conventionally treated with split-thickness skin autografts or epidermal sheets. However, neither early complete healing nor quality of epithelialization is satisfactory. An alternative approach is to graft isolated keratinocytes. ⋯ We report a successful method for grafting paediatric males presenting large severe burns through direct spreading of autologous foreskin keratinocytes. This alternative method is easy to implement, improves the quality of skin and minimizes associated donor site morbidity. In vitro studies have highlighted the potential of foreskin tissue for graft applications and could help in tissue selection with the prospect of grafting burns for girls.
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J Tissue Eng Regen Med · Mar 2016
Demineralized bone matrix and human cancellous bone enhance fixation of porous-coated titanium implants in sheep.
Allogenic bone graft has been considered the gold standard in connection with bone graft material in revision joint arthroplasty. However, the lack of osteogenic potential and the risk of disease transmission are clinical challenges. The use of osteoinductive materials, such as demineralized bone matrix (DBM), alone or in combination with allograft or commercially available human cancellous bone (CB), may replace allografts, as they have the capability of inducing new bone and improving implant fixation through enhancing bone ongrowth. ⋯ We found inferior early effects of DBM alone on the fixation of porous-coated titanium implant in this animal model, while the long-term effects have to be investigated. The combination of DBM with CB, which can be used off the shelf, may represent an alternative to allograft. A cost-benefit analysis is necessary before application in clinical trial.