American journal of rhinology & allergy
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Am J Rhinol Allergy · Sep 2012
The use of nasal epithelial stem/progenitor cells to produce functioning ciliated cells in vitro.
Although epithelial stem/progenitor cells have been isolated from many parts of the human airway epithelium such as lung and trachea, there is limited information in regard to stem cells in nasal epithelium. The aim of this study was to determine if (1) human nasal epithelial stem/progenitor cells (hNESPCs) can be isolated and propagated in vitro and (2) allogeneic adult primary human fibroblasts can serve as a feeder layer for hNESPCs expansion under serum-free conditions. ⋯ Our results showed the feasibility of expanding hNESPCs for clinical purpose by using human feeder layer, avoiding components of animal source, while preserving their self-renewal and differentiation potential. This study represents an early step toward a better understanding of hNESPCs, and serum -free media plus human feeder potentially would be an ideal method for making clinical grade hNESPCs on a large scale.
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Am J Rhinol Allergy · Sep 2012
Randomized Controlled TrialAdvantages of glove finger-coated polyvinyl acetate pack in endoscopic sinus surgery.
This study investigated the efficacy of glove finger-coated polyvinyl acetate (PA) pack on hemostasis, pain levels, and wound healing after endoscopic sinus surgery (ESS). ⋯ PA packing in a glove finger is advantageous in terms of pain, bleeding on packing removal, and postoperative wound healing, compared with PA pack only.