Medicine
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This study aimed to explore the mode of action of Yiqiyangyinquyu prescription (YP) against Sjögren's syndrome (SS) by combining network pharmacology with molecular docking techniques. YP's active components and target proteins were identified using the BATMAN-traditional Chinese medicine database. Concurrently, targets associated with SS were extracted from databases, including Genecards, Online Mendelian Inheritance in Man, and Therapeutic Target Database. ⋯ Noteworthy, TNF-α, considered the most important gene in YP against SS, binds to YP most stably, which was further validated by molecular dynamics simulation. In vitro experiments confirmed YP's capacity to reduce TNF-α, IL-1β, and IL-6 expression, effectively alleviating SS-related inflammation. YP demonstrated a significant anti-inflammatory effect by suppressing inflammatory cytokines (TNF-α, IL-6, and IL-1β), providing experimental evidence for its clinical application in treating SS.
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As a product of glycolysis, lactate contributes to cancer proliferation, immunosuppression, and metastasis via histone lactylation. However, the relationship between cutaneous melanoma (CM) and lactylation-associated genes and lncRNAs has remained unclear. In this study, 4 mechanism learning algorithms and integrated bioinformatic analyses were employed to identify the core lactylation-associated genes and lncRNAs. ⋯ Furthermore, mechanistic analyses suggested a significant association between the risk signature and the immune microenvironment in CM, highlighting potential immune-related implications in disease progression. In conclusion, we propose that lactylation-associated genes and lncRNAs hold promise as potential targets in CM. Moreover, our findings revealed a significant correlation between lactylation and the immune microenvironment, providing crucial insights for guiding individualized treatment strategies in CM.
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The objective of this research was to evaluate the impact of an empathy-centered care approach on the intensity of negative emotional states and levels of expectation in patients experiencing acute heart failure. A retrospective analysis was conducted on the clinical data of 106 patients presenting with emergent heart failure. The patients were bifurcated into a control group (53 patients) and an intervention group (53 patients) based on their respective care management plans. ⋯ Additionally, the 6-minute walk test used for cardiopulmonary rehabilitation and the 30 seconds sit-to-stand exercise revealed superior results in the intervention group (P < .05). Positive scores on the Positive Negative Affect Scale, the various dimensions of the Herth Hope Index Scale, the Psychological Resilience Scale, and the Chinese Cultural Modified Minnesota Living with Heart Failure Questionnaire were notably higher in the intervention group, whereas negative scores on the Positive Negative Affect Scale and scores on the Self-Assessment Scale of Anxiety were comparatively lower than those in the control group (P < .05). Implementing an empathy-based care approach can bolster cardiac function, augment functional fitness, mitigate negative emotional states, elevate expectation levels, enhance psychological resilience, improve quality of life, and decrease complication rates in patients with acute heart failure.
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Lung cancer is one of the most common cancers in China and has a high mortality rate. Most patients who are diagnosed have lost the opportunity to undergo surgery. Aberrant metabolism is closely associated with tumorigenesis. We aimed to identify an effective metabolism-related prediction model for assessing prognosis based on the cancer genome atlas (TCGA) and GSE116959 databases. ⋯ In this study, we constructed a DEMRGs signature and established a prognostic nomogram that is robust and reliable to predict OS in LUAD. Overall, the findings could help with therapeutic customization and personalized therapies.
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Cerebral ischemia-reperfusion injury (CIRI) is a complex pathophysiological process that typically occurs during the treatment of ischemia, with limited therapeutic options. Autophagy plays a vital role during the reperfusion phase and is a potential therapeutic target for preventing and treating cerebral ischemia-reperfusion injury. ⋯ The role of autophagy in cerebral ischemia-reperfusion injury remains unclear, and the specific mechanisms of drugs used to treat ischemia-reperfusion injury still need to be explored. This work outlines the changing trends in investigating cerebral ischemia-reperfusion injury involving autophagy and suggests future lines of inquiry.