Journal of clinical microbiology
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J. Clin. Microbiol. · Sep 2012
Investigation of false-positive results given by the QuantiFERON-TB Gold In-Tube assay.
We investigated a sudden increase in the rate of positive QuantiFERON-TB Gold In-Tube results from 10% to 31% at a U. S. academic institution. ⋯ The underlying defect remains unknown. This finding warrants refinement of quality control programs by the manufacturer and users.
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J. Clin. Microbiol. · Sep 2012
Recurrent bacteremia caused by the Acinetobacter calcoaceticus-Acinetobacter baumannii complex.
This study investigated the clinical and microbiological characteristics of patients with recurrent bacteremia caused by the Acinetobacter calcoaceticus-Acinetobacter baumannii (ACB) complex at a medical center. All ACB complex isolates associated with recurrent bacteremia were identified to the genomic species level using a 16S-23S rRNA gene intergenic spacer sequence-based method. Genotypes were determined by the random amplified polymorphic DNA patterns generated by arbitrarily primed PCR and by pulsotypes generated by pulsed-field gel electrophoresis. ⋯ A. baumannii isolates had lower antimicrobial susceptibility rates than A. nosocomialis and A. pittii isolates. In conclusion, relapse of ACB complex bacteremia can develop in immunocompromised patients, especially those with central venous catheters. Molecular methods to identify the ACB complex to the genospecies level are essential for differentiating between reinfection and relapse of bacteremia caused by the ACB complex.