Biochemical Society transactions
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Biochem. Soc. Trans. · Feb 2020
ReviewEditor's cut: DNA cleavage by CRISPR RNA-guided nucleases Cas9 and Cas12a.
Discovered as an adaptive immune system of prokaryotes, CRISPR-Cas provides many promising applications. DNA-cleaving Cas enzymes like Cas9 and Cas12a, are of great interest for genome editing. ⋯ Although Cas nucleases are highly promising, some room for improvement remains. New developments and discoveries like base editing, prime editing, and CRISPR-associated transposons might address some of these challenges.