Neuroscience letters
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Neuroscience letters · Dec 1991
Sympathectomy alleviates mechanical allodynia in an experimental animal model for neuropathy in the rat.
We attempted to determine the effects of surgical sympathectomy on an animal model for neuropathic pain. The L5 and L6 spinal nerves on one side were tightly ligated in anesthetized rats. ⋯ The sympathectomy produced an immediate and almost complete reversal of the increased mechanical sensitivity, whereas sham sympathectomy had no effect. The data suggest that sympathectomy alleviates mechanical allodynia in this experimental animal model.
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Neuroscience letters · Dec 1991
Comparative StudyNon-NMDA antagonists protect against kainate more than AMPA toxicity in the rat hippocampus.
Single focal injection of the excitatory amino acids (EAAs) kainic acid (KA, 1.1 nmol/microliters) and (S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (S)-AMPA, 6 nmol/microliters) into rat dorsal hippocampus resulted in widespread neurodegeneration with 90-100% loss of hippocampal pyramidal cells in CA1, CA2, CA3 and CA4 subfields, and 50-70% loss of dentate granule (DG) cells. Focal injection of NMDA (30 nmol/microliters) under the same conditions resulted in 70-90% loss of CA1 cells with less damage in CA2, CA3, CA4 and DG cells (30-50%, 10-30%, and 30-50%, respectively). The non-NMDA antagonists NBQX (2,3-dihydro-6-nitro-7-sulphamoyl-benzo(f) quinoxaline) and GYKI 52466 (1-(amino)phenyl-4-methyl-7,8-methylendioxy-5H-2,3,benzodiazepine. ⋯ NBQX i.v. protected against (S)-AMPA toxicity in the DG cells but no protection was observed against (S)-AMPA toxicity in hippocampal subfields (CA1, CA2 and CA4). Intravenous administration of NBQX and GYKI 52466 (30 mg/kg/3 h) also failed to protect against NMDA toxicity in the hippocampus. Systemic injections of D(-)-CPPene, (E)-4-(3-phos-phonoprop-2-enyl)-piperazine-2-carboxylic acid, (10 and 5 mg/kg, i.p., 20 min prior and 3 h post EAA injection) protected against NMDA and KA toxicity in the CA1, CA2 and DG subfield with no protective effect against (S)-AMPA toxicity.