Neuroscience
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Two event-related potential (ERP) experiments were conducted to investigate whether Cantonese lexical tones are processed with general auditory perception mechanisms and/or a special speech module. Two tonal features (f0 direction and f0 height deviation) were manipulated to reflect acoustic processing, and the contrast between syllables and hums was used to reveal the involvement of a speech module. Experiment 1 adopted a passive oddball paradigm to study a relatively early stage of tonal processing. ⋯ Moreover, detecting tonal deviants of syllables elicited N1 and P2 that were not found in hum detection. Together, these findings suggest that the processing of lexical tone relies on both acoustic and linguistic processes from the early stage. Another noteworthy finding is the absence of brain lateralization in both experiments, which challenges the use of a lateralization pattern as evidence for processing lexical tones through a special speech module.
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The development of a hyperexcitable neuronal network is thought to be a critical event in epilepsy. Thrombospondins (TSPs) regulate synaptogenesis by binding the neuronal α2δ subunit of the voltage-gated calcium channel. TSPs regulate synapse formation during development and in the mature brain following injury. ⋯ Here we report similar findings, TSP1, and TSP1/2 KO mice have low levels of CACNA2D2 mRNA expression and α2δ-1/2 receptor level in the cortex, and are more susceptible to seizures. CACNA2D2 mutations in mice and humans can cause epilepsy. Our data suggest TSP1 in particular may control CACNA2D2 levels and could be a modifier of seizure susceptibility.
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Recent functional magnetic resonance imaging (fMRI) studies consistently revealed contributions of fronto-parietal and related networks to the execution of a visuospatial judgment task, the so-called "Clock Task". However, due to the low temporal resolution of fMRI, the exact cortical dynamics and timing of processing during task performance could not be resolved until now. In order to clarify the detailed cortical activity and temporal dynamics, 14 healthy subjects performed an established version of the "Clock Task", which comprises a visuospatial task (angle discrimination) and a control task (color discrimination) with the same stimulus material, in an electroencephalography (EEG) experiment. ⋯ We divided this window in two parts: an early phase - from 192 ms to ∼440 ms, and a late phase - from ∼440 ms to 776 ms. For both tasks, the order of network activations and the types of networks were the same, but, in each phase, activations for the two conditions were dominated by differing network states with divergent temporal dynamics. Our results provide an important basis for the assessment of deviations in processing dynamics during visuospatial tasks in clinical populations.
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Alzheimer's disease (AD) is a progressive neurodegenerative disorder and the most common type of dementia among older people. The number of patients with AD will grow rapidly each year and AD is the fifth leading cause of death for those aged 65 and older. In recent years, one of the main challenges for medical investigators has been the early diagnosis of patients with AD because an early diagnosis can provide greater opportunities for patients to be eligible for more clinical trials and they will have enough time to plan for future, medical and financial decisions. ⋯ We selected 50 percent of the MRIs randomly for use in training the classifiers, 25 percent for validation and we used 25 percent for the testing phase. The technique proposed here yielded the best overall classification results between AD and MCI (accuracy 94.88%, sensitivity 94.18%, and specificity 95.55%), and for pairs of the MCI and HC (accuracy 95.59%, sensitivity 95.89% and specificity 95.34%). These results were achieved using maximum order 30 of PZM and the pattern recognition network with the scaled conjugate gradient (SCG) back-propagation training algorithm as a classifier.
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The role of microRNAs (miRNAs) in lysosome-mediated neuronal death and survival following ischemic stroke remains unknown. Herein, using miRNA and mRNA gene expression profiling microarrays, we identified the differentially expressed 24 miRNAs and 494 genes in the cortical peri-infarct area, respectively. Integrating the miRNA targets and mRNA expression profiles, we found 47 genes of miRNA targets, including lysosomal-associated membrane protein 2 (LAMP2), Hexb, Bcl2, etc. ⋯ In addition, miR-207 mimics could reduce the number of cellular lysosome and autophagosome, whereas increase the number of autophagic vacuoles, indicating miR-207 might affect the latter part of lysosomal-autophagy pathway and mitochondria-induced apoptosis. These results suggested that miR-207 and miR-352 were involved in lysosomal pathway for mediating ischemic injury and spontaneous recovery. MiR-207 mimics as potential target drugs could protect against autophagic cell death after ischemic stroke.