Neuroscience
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This study was aimed to investigate frequency-specific LFO changes and their correlation with gene pathways in PACG using transcriptome-neuroimaging analysis. ⋯ This study establishes a connection between the molecular mechanisms of PACG and alterations in brain function and gene expression, providing valuable perspectives on the fundamental processes impacting low-frequency oscillations in PACG.
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It is common knowledge that the cerebellum is a structure of the central nervous system that influences the processes of balance and motor coordination. Recently its influence on social interactions has also been emphasized. The sigma receptor agonist: 3-di-o-tolylguanidine (DTG) is characterized by high affinity for sigma 1 and sigma 2 receptors, widely distributed in the cerebellum. ⋯ At the same time, it was shown that in the group receiving the lowest dose of the drug, a decrease in the concentration of dopamine and serotonin in the cerebellum was observed. Furthermore, changes in the concentration of taurine, alanine, glutamic acid and gamma-aminobutyric acid were observed in the treated groups. We found that long term administration of DTG disturbs animals' social interactions and the concentration of neurotransmitters in the cerebellum.
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Our previous in silico data indicated an overrepresentation of the ZF5 motif in the promoters of genes in which circadian oscillations are altered in the ventral hippocampus in the pilocarpine model of temporal lobe epilepsy in mice. In this study, we test the hypothesis that the Zbtb14 protein oscillates in the hippocampus in a diurnal manner and that this oscillation is disrupted by epilepsy. We found that Zbtb14 immunostaining is present in the cytoplasm and cell nuclei. ⋯ In the pilocarpine model of epilepsy, an increase in the level of Zbtb14 protein was found at 11 PM but not at 3 PM compared to controls. Finally, in silico analysis revealed the presence of the ZF5 motif in the promoters of 21 out of 24 genes down-regulated by epileptiform discharges in vitro, many of which are involved in neuronal plasticity. Our data suggest that Zbtb14 may be involved in the diurnal dynamic of seizure regulation or brain response to seizure rhythmicity.