Indian journal of pathology & microbiology
-
Indian J Pathol Microbiol · Oct 2000
Polymerase chain reaction using IS6110 primer to detect Mycobacterium tuberculosis in clinical samples.
Nucleic acid amplification using IS6110 primers to detect Mycobacterium tuberculosis in clinical specimens has been extensively used as laboratory tool for the diagnosis for tuberculosis. Despite it's dramatic scientific value in practice, it is not as sensitive as expected for the detection of Mycobacterium tuberculosis. The results of the study suggest that PCR using 123 bp fragment of DNA belonging to IS6110 is specific (95.6%) but only has a sensitivity of 30% to detect M. tuberculosis in clinical specimens.
-
External quality assessment forms a vital aspect of health laboratory service. Decisionto start National External quality assessment Programme (NEQAP) under IAPM was taken at the Annual conference at Bombay in December, 1989 to be run from Varanasi at the initiative of the author. ⋯ It covers about 300 labs from all corners of India. Details of the IAPM-NEQAP programme made during successful running for ten years (1990-1999) and recommendations for further action are given.