Archives of pathology & laboratory medicine
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Arch. Pathol. Lab. Med. · Mar 2010
Quantitative real-time polymerase chain reaction detection of BK virus using labeled primers.
BK virus infections among immunocompromised patients are associated with disease of the kidney or urinary bladder. High viral loads, determined by quantitative polymerase chain reaction (PCR), have been correlated with clinical disease. ⋯ Real-time PCR using labeled primers is highly sensitive and specific for the quantitative detection of BK virus from a variety of clinical specimens. These data demonstrate the applicability of labeled primer PCR for quantitative viral detection and offer a simplified method that removes the need for separate oligonucleotide probes.