Pathologie-biologie
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Pathologie-biologie · Oct 2011
Characterization of Pseudomonas aeruginosa strains isolated from cystic fibrosis patients by different typing methods.
Pseudomonas (P.) aeruginosa strains isolated from the sputum of cystic fibrosis patients (CFP) are frequently difficult to type by conventional typing methods. The purpose of this study was to develop a random amplified polymorphic DNA (RAPD) analysis for the routine typing of these strains. Sixty P. aeruginosa non-repetitive strains recovered from CFP in a teaching hospital were typed. ⋯ Strains belonging to the other cluster were present in three (8%) of the 35 strains. The occurrence of these clusters indicates that cross-infection may occur. The results indicate also that only the RAPD method can establish a clonal relation whereas the other methods may only reflect phenotypical differences, and thus are inadequate to type these strains.