Sleep
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The present paper has three major objectives: first, to document the reliability of a published criteria set for sleep/wake scoring in the rat; second, to develop a computer algorithm implementation of the criteria set; and third, to document the reliability and functional validity of the computer algorithm for sleep/wake scoring. The reliability of the visual criteria was assessed by letting two raters separately score 8 hours of polygraph records from the light period from five rats (14,040 10-second scoring epochs). Scored stages were waking, slow-wave sleep-1, slow-wave sleep-2, transition type sleep and rapid eye movement (REM) sleep. ⋯ The computer scoring algorithm was applied to data from a third independent group of rats (n = 6) from an acoustical stimulus arousal threshold experiment, to assess the functional validity of the scoring directly with respect to arousal threshold. The computer algorithm scoring performed as well as the original visual sleep/wake stage scoring. This indicated that the lower intrarater reliability did not have a significant negative influence on the functional validity of the sleep/wake score.