Journal of neuroscience methods
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J. Neurosci. Methods · Sep 1987
Specific, selective, and complete staining of neurons of the myenteric plexus, using cuprolinic blue.
Wholemounts consisting of both muscular layers of the rat's intestine have been stained with Cuprolinic blue and magnesium chloride. The procedure, which is specific for RNA, gives selective coloration of the somata of the neurons of the myenteric plexus. ⋯ The absence of background color enables neurons to be identified more confidently and counted more quickly than is possible with other techniques, in which nuclei or mitochondria in smooth muscle and neuroglial cells are also stained. Numbers of myenteric neurons determined using the new method are higher than those obtained by other means.