Journal of neuroscience methods
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J. Neurosci. Methods · Oct 2013
Superparamagnetic beads for estimation of spinal subarachnoid space permeability in rats.
Human spinal pathological processes have been linked to a loss of spinal subarachnoid space (SSAS) permeability, which has therefore become a target for therapy. Hence, it has become important to measure SSAS patency in rat models of these human disorders. ⋯ A novel method has been established to reliably determine SSAS permeability in rats. The method is reproducible and has the required sensitivity to detect an 84.4% reduction in bead recovery, as seen in laminectomized rats compared to intact animals.
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Many studies have investigated test-retest reliability of active voxel classification for fMRI, which is increasingly important for emerging clinical applications. The implicit impact of voxel-wise thresholding on this type of reliability has previously been under-appreciated. This has had two detrimental effects: (1) reliability studies use different fixed thresholds, making comparison of results is challenging; (2) typical studies do not assess reliability at the individual level, which could provide information for selecting activation thresholds. ⋯ This variation depended strongly on the individual tested. Finally, we demonstrated novel procedures using ROC-r and overlap analysis to optimize thresholds on a case-by-case basis. Ultimately, a method to determine robust individual-level activation maps represents a critical advance for fMRI as a diagnostic tool.