The Journal of neuroscience : the official journal of the Society for Neuroscience
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In the preceding companion article (Berkowitz and Stein, 1994b), we showed that many descending propriospinal neurons in the turtle were rhythmically activated during two different motor patterns, fictive rostral scratching and fictive pocket scratching. In this article, we present phase analyses of the activity of each such neuron during fictive scratching. Each neuron's activity was concentrated in a particular phase of the ipsilateral hip flexor muscle nerve (VP-HP) activity cycle; each had a distinct "preferred phase." Each neuron's preferred phase during fictive rostral scratching was similar to its preferred phase during fictive pocket scratching. ⋯ Thus, the selection of an appropriate motor pattern and the production of the required knee-hip synergy may each be distributed over a diverse population of spinal cord neurons. This model requires that each such neuron project to both knee muscle and hip muscle motoneurons. According to this model, the process of selecting a motor pattern would not be completed until knee muscle motoneurons integrate overlapping excitatory and inhibitory inputs.
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We recorded the activity of descending propriospinal axons at the caudal end of a seven-segment (D3-D9) turtle spinal cord preparation. These seven spinal segments contain sufficient neural circuitry to select and generate fictive rostral scratching or fictive pocket scratching in response to tactile stimulation in the appropriate region of the body surface. Each turtle received two spinal transections, one just caudal to the forelimb enlargement and one in the middle of the hindlimb enlargement. ⋯ Thus, these units were broadly tuned to a region of the body surface. Some were tuned to a region of the rostral scratch receptive field and others were tuned to a region of the pocket scratch receptive field. These data suggest that selection of the appropriate form of scratching, rostral or pocket, may be mediated by populations of broadly tuned neurons rather than by highly specialized neurons.
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The role of spinal voltage-sensitive calcium channels (VSCC) in a behavioral model of prolonged nociception was examined in rats. Blockade of VSCC by the trivalent cations neodymium (NdCl3) and lanthanum (LaCl3) resulted in a dose-dependent suppression of both phases of the response to formalin. omega-Conopeptides, which selectively block N-type VSCC, also produced a dose-dependent inhibition of both the initial behavior [phase 1; ED50 (nmol): SNX-111 (0.003) > SNX-185 (0.010) > SNX-239 (0.16) > SNX-159 (> 0.26); SNX-199 (> 0.30)] and the facilitated response [phase 2; ED50 (nmol): SNX-111 (0.003) > SNX-185 (0.009) > SNX-239 (0.020) > SNX-159 (0.120) = SNX-199 (0.230)]. In contrast, SNX-231 (0.24 nmol), which is selective for a non-L/non-N site and also the L-type VSCC blockers nifedipine (24 nmol), nimodipine (29 nmol), verapamil (200 nmol), and diltiazem (220 nmol), had minimal effects on either phase of the formalin test at the highest dose examined. ⋯ High doses of the N-type VSCC produced characteristic shaking behavior, serpentine-like tail movements, and impaired coordination. However, at antinociceptive doses there was no significant motor effect, though three of the N-type antagonists produced some tail movements. These studies demonstrate that VSCC of the N- and P-type, but not L-type, are involved in facilitated nociceptive processing at the spinal level.