Neuroscience research
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Neuroscience research · Jun 1994
Comparative StudyDistribution of corticotropin-releasing factor and calcitonin gene-related peptide in the developing mouse cerebellum.
Corticotropin-releasing factor (CRF)-like immunoreactive (IR) fibers were investigated ontogenically in the mouse cerebellum. CRF-IR was detected in the climbing fiber and mossy fibers as in other species. In addition, CRF-IR dense fiber plexuses were detected from postnatal day (PD) 2 to 9, in the developing Purkinje cell layer of the vermal lobules, paraflocculus, flocculus and crus 1 ansiform lobule, gradually forming a pericellular nest around the Purkinje cell somata. ⋯ No neurons containing both CRF and CGRP immunoreactivities were observed. These results suggest that CGRP- and CRF-IR developing climbing fibers innervate different compartments of Purkinje cells, especially in the vestibular cerebellar cortex in mice. Furthermore, CRF-IR fibers gradually changed to become typical climbing fibers, while CGRP-IR disappeared altogether.