Microbial pathogenesis
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Microbial pathogenesis · Sep 2014
Aspirin is an efficient inhibitor of quorum sensing, virulence and toxins in Pseudomonas aeruginosa.
Quorum sensing (QS) plays a vital role in regulation of virulence factors and toxins in Pseudomonas aeruginosa, which can cause serious human infections. Therefore, the QS system in P. aeruginosa may be an important target for pharmacological intervention. Activity of aspirin on the QS system was assessed using a reporter strain assay and confirmed using RT-PCR to test expression of virulence factors and toxins. ⋯ The molecular modeling analysis suggests the QS inhibitory action of aspirin occurs through interaction of aspirin's aryl group and Tyr-88 of the LasR receptor, by strong π-π stacking interactions, which associated with a conformational change of the receptor-aspirin complex. The inhibitory effect of aspirin on virulence factors was specific to P. aeruginosa as aspirin at sub-MIC did not affect the biofilm or motility of Escherichia coli. To summarize, the collective data demonstrate that low concentrations of aspirin inhibit quorum sensing of P. aeruginosa.