Rapid communications in mass spectrometry : RCM
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Rapid Commun. Mass Spectrom. · Jul 2014
Novel method to analysis benzo[a]pyrene in filter by liquid chromatography/tandem mass spectrometry: application to assess mouth level benzo[a]pyrene exposure.
Benzo[a]pyrene (B[a]P) is a well-known carcinogenic compound produced from incomplete combustion of organic compounds. During cigarette smoking, cigarette filters trap a portion of mainstream smoke B[a]P and accurate measurement of B[a]P levels in cigarette filter can be used to estimate human exposure level of this compound. ⋯ The LC/APPI-MS/MS method developed in this work had an excellent throughput in estimating the mouth-level exposure under natural human smoking conditions. Mouth-level B[a]P exposure based on a study of three cigarettes (8, 10 and 13 mg tar levels) showed significant positive correlations with B[a]P trapped by cigarette filter measured (y = 2.5456x - 0.5056, R(2) = 1, n = 249).