Prostaglandins, leukotrienes, and essential fatty acids
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Prostaglandins Leukot. Essent. Fatty Acids · Oct 2018
A stable method for routine analysis of oxylipins from dried blood spots using ultra-high performance liquid chromatography-tandem mass spectrometry.
Oxylipins are biologically important lipid mediators that are derived enzymatically from polyunsaturated fatty acids (PUFA) and have a major role in regulating inflammatory processes. The currently available methods for measuring oxylipins from human biological samples have limitations, which restricts their use in large studies. We have developed a novel method for measuring 21 oxylipins from dried blood spot (DBS) using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) and stable isotope dilution analysis. ⋯ Recovery of oxylipins from the DBS ranged from 80 - 115%. The 12 spiked oxylipins were stable for 2 months when stored as DBS at room temperature. Our method is reproducible and precise, and provides the opportunity to accurately quantitate these oxylipins in a small sample volume.