Phytotherapy research : PTR
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Lamiophlomis rotata (Benth.) Kudo (LR) is a traditional drug used by Chinese minorities such as Tibetans, Mongolians and Na Xi with the beneficial effects of hemostasis and alleviating pain. Flavonoids ingredients (P(1)), iridoid glycosides ingredients (P(2)) and maximus polarity components ingredients (P(3)) were isolated from the aqueous extract of LR (AELR) with polyamide and macroporous adsorptive resins chromatographic columns. The hemostatic activity of these ingredients was estimated by the changes in bleeding time (BT), clotting time (CT) and blood coagulation parameters in mice or rats. ⋯ As a result, AELR and P(2) significantly shortened BT, CT and thrombin time (TT), and increased fibrinogen (FIB), but P(1) and P(3) showed no hemostatic effect during the experiment. Moreover, P(2) showed satisfactory hemostatic effect with dose-effect relationship and low toxicity in mice. 8-Dehydroxy shanzhiside, phloyoside II, shanzhiside methylester, loganin, and 8-O-acetylshanzhiside methylester were detected as the main iridoid glycosides in P(2) by RP-HPLC. Our results suggest that iridoid glycosides may be the hemostatic activity ingredients of L. rotata and are a potential hemostatic medicine.
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Sepsis continues to be a challenge in clinic. Therapeutic strategies focus on local host defenses and the inhibition of overwhelming inflammation response. The present study aimed to investigate the protective effects and the underlying mechanisms of the ethanol extract of Alpinia katsumadai Hayata seeds (EAKH) on polymicrobial sepsis induced by cecal ligation and puncture (CLP) in mice. ⋯ Although EAKH was devoid of direct bacteriostatic or bacteriocidal activities, it facilitated peritoneal bacteria clearance and increased leukocyte migration into peritoneal cavity of septic mice. Furthermore, EAKH remarkably decreased serum pro-inflammatory cytokine (TNF-alpha, IL-1beta and NO) levels in septic mice. These findings demonstrated that EAKH has preventive effects on mouse sepsis induced by CLP, which may be attributed to elevating local defense via promoting leukocyte migration to infection focus and attenuating systemic inflammation.
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Randomized Controlled Trial
Effect of pine bark extract (Pycnogenol) on symptoms of knee osteoarthritis.
The safe and efficacious use of Pycnogenol (French maritime pine bark extract) in other inflammatory diseases prompted this study of its antiinflammatory effects in patients with osteoarthritis (OA). The aim of the study was to evaluate whether Pycnogenol reduces the symptoms of OA in a double-blind, placebo-controlled, randomly allocated trial with patients suffering from knee osteoarthritis stages I and II. ⋯ Results show that Pycnogenol in patients with mild to moderate OA improves symptoms and is able to spare NSAIDs.
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To date most pharmacological studies on mistletoe (Viscum album L.) have focused on the therapeutic properties of its polar extracts. This study examined the non-polar constituents of Viscum album and their biological activities. Supercritical CO(2) extraction coupled with gas chromatography/mass spectrometry (GC/MS) was used to selectively extract and identify compounds in Viscum album leaves. ⋯ The largest decrease was observed in mice pretreated with the Viscum album extract, although significantly reduced numbers of EAC cells were also observed in animals with developed carcinoma. The activities of antioxidative enzymes in the EAC cells suggested the absence of oxidative stress. However, changes in the antioxidative enzymes activities observed after administration of the Viscum album extract might be due to the induction of oxidative stress in the EAC cells.
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The blood hemostatic activity of the Tibetan medicinal herb Lamiophlomis rotata was evaluated in BALB/c mice and Wistar rats. L. rotata aqueous extract (LRAE) was given to mice at concentrations of 0.5, 1.0, 2.0 g/kg body weight and 0.75, 1.5, 3.0 g/kg to rats. The hemostatic activity of LRAE was estimated by changes in bleeding time (BT), prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB). ⋯ On the other hand, LRAE showed some effects in improving the liver function and reducing blood lipids by decreasing the levels of AST, ALT, Chol and LDL-C. All these changes had a significant dose-effect and time-effect relationship. These results confirm the hemostatic and thromboplastic effects of L. rotata and these effects might be implemented by improving the synthetic function of the liver.