Scandinavian journal of medicine & science in sports
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Scand J Med Sci Sports · Apr 2012
Comparative StudyDependence of hemoglobin mass estimation with the optimized CO-rebreathing method on different spectrophotometers.
The assessment of total hemoglobin mass (tHb-mass) with the optimized carbon monoxide-rebreathing procedure (oCOR) is discussed as a promising method to detect blood doping. The method requires repeated measurements of the carboxyhemoglobin fraction (%HbCO) using spectrophotometers (CO oximeters). In order to determine whether %HbCO measurements with different spectrophotometers yield similar tHb-masses, the results of 57 tHb-mass calculations from simultaneous %HbCO measurements with two different spectrophotometers (RapidLab, OSM3) were analyzed. ⋯ In only about 70% of all ΔtHb-mass estimations did ΔtHb-mass(OSM3) and ΔtHb-mass(RapidLab) show the same direction of change. Apparently, the analytical variation in tHb-mass determination with oCOR increases considerably with the use of different spectrophotometers. Therefore, agreement on the use of one spectrophotometer that accurately measures low %HbCO values is needed if oCOR should be used in an anti-doping setting.